WebJul 29, 2014 · Phospho-p38α MAPK, p38MAPK antibodies and SB202490 were purchased from Cell Signaling (Danvers, MA). Casein kinase 2 (CK2) antibody, human IGFBP-3 immunoassay ELISA kit, and TNFα were purchased from R & D (Minneapolis, MN). Phospho-CK2 antibody was purchased from Sigma-Aldrich (St. Louis, MO). BIRB 796 was … WebConstitutively phosphorylated by CK2 under normal conditions. Phosphorylated in vitro by MAST1, MAST2, MAST3 and STK11. Phosphorylation results in an inhibited activity towards PIP3. ... Anti-PTEN (phospho S370) antibody (ab195056) Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PTEN (phospho S370) with ...
(8738) Phospho-CK2 Substrate [(pS/pT)DXE] MultiMab - CiteAb
WebOct 8, 2007 · Here we describe a new phospho-specific antibody against Cdc37 that recognizes recombinant purified Cdc37 only when incubated with CK2 in the presence of Mg 2+ and ATP. The replacement of Ser13 in Cdc37 by nonphosphorylatable amino acids abolished binding to this antibody. WebNov 1, 2007 · A new phospho‐specific antibody against Cdc37 is described that recognizes recombinant purified CDC37 only when incubated with CK2 in the presence of Mg2+ and ATP and showed that complexes of Hsp90 with its client signaling kinases, Cdk4, MOK, v‐Src, and Raf1, contained the CK2‐phosphorylated form of C dc37 in vivo. The … dwc form sbr 1
Phospho-Bad (Ser136) (5D8) Mouse mAb Cell Signaling …
WebDec 22, 2024 · CK2 kinase activity is often determined by measuring the phosphorylation of one of the down-stream signaling molecules, namely Akt, which is phosphorylated by CK2 at serine129 . Phosphorylation of Akt is detected by a phospho-specific antibody directed against the CK2 phosphorylation site serine 129. WebThe antibody was specific for phosphorylated Cdc37 and did not crossreact with other CK2 substrates such as Hsp90 and FK506-binding protein 52. Using this antibody, we showed … WebCasein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, … crystal fries food truck