Phis2载体图谱
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Phis2载体图谱
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WebbpHis2酵母表达载体说明 pHis2 编号 北京华越洋生物VECT2110 pHis2载体基本信息 出品公司: 载体名称: 质粒类型: 高拷贝/低拷贝: 启动子: 克隆方法: 载体大小: 5' 测序引物及序列: … WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点,限制性内切酶: 载体大小: 7207bp: 5 测序引物及序列:--3 测序引物及序列:--载体标签:--载体抗性: 卡 …
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WebbDescription pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … WebbTo use pHIS2 in a one-hybrid assay, clone one or more copies of a cis-acting DNA target sequence into the MCS. Then introduce the plasmid into competent yeast cells using the …
WebbThe concentration of 3-AT needed to fully suppress leaky HIS3 expression must be determined empirically for each DNA target element.pHIS2 can be maintained in both …
Webb1 Design Better Procedures Accurately design and simulate cloning procedures. Test complicated projects, catch errors before they happen, and obtain the right constructs … cryptographic services adalahcryptographic services bughttp://www.biofeng.com/zaiti/jiaomu/pHis2.html cryptographic services la giWebb诱饵载体:pHis2-DNA(筛选标记Trp报告基因His) 文库载体:pGADT7-cDNA(筛选标记Leu) 实验步骤: 一、转化诱饵载体(参考KD0256中质粒转化步骤) 1.YPDA平板培养划线,活 … dusk to dawn led outside pole barn lightsWebb20 aug. 2012 · (PR32049;publishedFebruary2003)pHIS2VectorInformationPT3705-5SoldCatalog#K1617 … cryptographic services enable windows 11Webb18 okt. 2024 · 载体描述: pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically … dusk to dawn light bulbs amazonWebbpet-30a (+)图谱 以利用T7 RNA聚合酶转录的载体pet-30a (+)为例,其包括的元件有: ① 复制起始点ori ② Bom ③ Rop ④ lacI promoter + lac I ⑤ T7 promoter ⑥ Lac operator ⑦ … cryptographic services engine csec